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comp_for comp_iter comp_if

5.2.6. Generator expressions

A generator expression is a compact generator notation in parentheses:

A generator expression yields a new generator object. Its syntax is the same as for comprehensions, except that it is enclosed in parentheses instead of brackets or curly braces.

Variables used in the generator expression are evaluated lazily when the method is called for generator object (in the same fashion as normal generators). However, the leftmost 2018 Summer Women Gladiator Shoes 10cm Block High Heels Ebay For Sale Cheap Wholesale Price Discounts Sale Online a1q9R0ZeTs
clause is immediately evaluated, so that an error produced by it can be seen before any other possible error in the code that handles the generator expression. Subsequent clauses cannot be evaluated immediately since they may depend on the previous loop. For example: .

The parentheses can be omitted on calls with only one argument. See section Calls for the detail.

generator_expression

5.2.7. Dictionary displays

A dictionary display is a possibly empty series of key/datum pairs enclosed in curly braces:

A dictionary display yields a new dictionary object.

If a comma-separated sequence of key/datum pairs is given, they are evaluated from left to right to define the entries of the dictionary: each key object is used as a key into the dictionary to store the corresponding datum. This means that you can specify the same key multiple times in the key/datum list, and the final dictionary’s value for that key will be the last one given.

A dict comprehension, in contrast to list and set comprehensions, needs two expressions separated with a colon followed by the usual “for” and “if” clauses. When the comprehension is run, the resulting key and value elements are inserted in the new dictionary in the order they are produced.

Restrictions on the types of the key values are listed earlier in section XYR Cheap Fashion Orange SlipOn Rubber Sole Vulcanized Shoe Buy Cheap Discount Outlet Cheap Price Discount Codes Shopping Online Limited Edition Sale Online 2018 Newest IF75r
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, which excludes all mutable objects.) Clashes between duplicate keys are not detected; the last datum (textually rightmost in the display) stored for a given key value prevails.

dict_display key_datum_list key_datum dict_comprehension

5.2.8. Set displays dolunke Casual Wedge Bodybuilding Canvas Shoes Women Lowest Price For Sale opzuxz94w4

A set display is denoted by curly braces and distinguishable from dictionary displays by the lack of colons separating keys and values:

A set display yields a new mutable set object, the contents being specified by either a sequence of expressions or a comprehension. When a comma-separated list of expressions is supplied, its elements are evaluated from left to right and added to the set object. When a comprehension is supplied, the set is constructed from the elements resulting from the comprehension.

An empty set cannot be constructed with ; this literal constructs an empty dictionary.

set_display

To further understand the molecular mechanisms of ISC self-renewal and differentiation, we carried out a transgenic RNAi screen and identified that the Osa-containing SWI/SNF complex regulates Drosophila ISC commitment to differentiation into discrete lineages. Loss-of-function mutations of subunits of the Osa-containing SWI/SNF complex resulted in ISC-like cell expansion at the expense of differentiated EC and EE cells. We demonstrated that Osa binds to the promoters of Dl and ase to regulate their expression, thus controlling ISC self-renewal and commitment to differentiation into EC and EE cells.

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Fly strains

The following fly strains were used: (Shigeo Hayashi, Riken); (Stephen DiNardo, University of Pennsylvania); (Francois Schweisguth, CNRS); (Bruce Edgar, University of Heidelberg); (Ken Irvine, Rutgers); (Mark Fortini, Thomas Jefferson University); (Sarah Bray, University of Cambridge); (Yuh Nung Jan, UCSF); (Tzumin Lee, Janelia Farm); (James Kennison, NIH); and (Andrew Dingwall, Loyola University). and fly strains used for MARCM clones (; >; ; ) were obtained from the Bloomington Drosophila Stock Center (BDSC) at Indiana University. The following transgenic RNAi lines were obtained from BDSC or the Vienna Drosophila RNAi Center (VDRC): (V7810 and BL31266), (V12645, V108599 and BL32372), (V37721 and BL31712), (V6969 and V110712) and (V108511).

The transgene was generated by cloning full-length cDNA of into pUAST ( Free Shipping Shop RAZAMAZA High Heel Sandals Platform Buckle Party Wiki Online Choice Cheap Online BawRG1vY
), and injecting purified DNA into the embryo using standard protocols.

MARCM clone assay

To induce MARCM clones of (as a wild-type control), and , we generated the following flies: >. The genotype of flies used to generate and double-mutant clones is >/. Three- or four-day-old adult female flies were heat shocked twice at an interval of 8-12 hours, at 37°C for 60 minutes. The flies were transferred to fresh food daily after the final heat shock, and their posterior midguts were processed for staining at the indicated times.

RNA interference (RNAi)-mediated gene depletion

Four male transgenic flies were crossed with eight virgin / or / virgin female flies at 18°C. One-week-old adult progenies of the correct genotype were transferred to new vials at 29°C for 7 days before dissection.

Quantitative PCR (qPCR) and chromatin immunoprecipitation (ChIP) assay

Total RNA from adult guts was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion to remove genomic DNA. cDNA was synthesized using the ThermoScript RT-PCR system (Invitrogen). Real-time PCR analysis was performed on the Mastercycler Realplex real-time PCR system (Eppendorf) using SYBR Green PCR Master Mix (Clontech). qPCR results are represented as mean ± s.e.m. of three biological replicates. Primer pairs for qPCR are listed in supplementary material Table S1.

Two hundred fly intestines were dissected for ChIP assays using the Magna ChIP G Tissue Kit (Millipore) with some modification. The primer pairs used to detect immunoprecipitated DNA are listed in supplementary material Table S1.

Antibody production

Polyclonal rabbit antisera were raised against 6×His fusion proteins containing amino acids 2-180 of the Snr1 protein. To produce the 6×His fusion protein, a 537 bp fragment of was amplified by PCR using primers 5′-AGTA GAATTC GCACTGCAGACATACGGGGA-3′ and 5′-AGTA GCGGCCGC TCACTCTAGCTCCATGTCCAGTC-3′ (restriction sites underlined). The amplified fragments were cloned into the RI and I sites of PET-28a (+) (Novagen). 6×His-Snr1 fusion protein was expressed in BL21(DE3) pLysS, purified on Ni-NTA agarose columns (Qiagen) and used to immunize rabbits as described previously ( 2018 cheap wholesale espadrilles woman shoes Shop For Sale Online Quality For Sale Free Shipping Big Sale Buy Cheap Affordable 6bKylg0SYn
).

Histology and image capture

The fly intestines were dissected in PBS and fixed in PBS containing 4% formaldehyde for 20 minutes. After three 5-minute rinses with PBT (PBS + 0.1% Triton X-100), the samples were blocked with PBT containing 5% normal goat serum overnight at 4°C. Then, the samples were incubated with primary antibody at room temperature for 2 hours and then with fluorescent secondary antibody for 1 hour at room temperature. Samples were mounted in Vectashield mounting medium with DAPI (Vector Laboratories). We used the following antibodies: mouse anti-β-Gal (1:200; Clontech); mouse anti-Dl [1:50; Developmental Studies Hybridoma Bank (DSHB)]; mouse anti-Pros (1:50; DSHB); nc82 (1:20; DSHB); rabbit polyclonal anti-Pdm1 (1:1000; a gift from X. Yang, Zhejiang University); rabbit anti-Spdo (1:1000; a gift from J. Skeath, Washington University in St Louis); mouse anti-Osa (1:20; DSHB); rabbit anti-Snr1 (1:1000; this study); rabbit anti-Ase (1:2000; a gift from Yuh Nung Jan); guinea pig anti-Sc (1:1000; a gift from S. Crews, UNC-Chapel Hill) and chicken anti-GFP (1:3000; Abcam). Secondary antibodies were goat anti-mouse, anti-chicken and anti-rabbit IgG conjugated to Alexa 488, Alexa 568 or Alexa 649 (1:400; Molecular Probes). Images were captured with a Zeiss LSM 510 confocal system and processed with LSM5 Image Browser (Zeiss) and Adobe Photoshop.

Quantification and statistical analysis

To quantify the number of () or Prospero (Pros) cells in Fig. 1 and Fig. 5 , the or Pros cells were counted in a 5×10 μm area of the field. In New womens street casual travelling espadrilles shoe Store Sale High Quality Sale Online Sale Factory Outlet Buy Cheap Original sRkZkL0l
, all of the images were taken with the same confocal settings and the fluorescence intensity was measured using LSM5 Image Browser. All data were analyzed using Student’s -test and sample size () is shown in the text.

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To identify new regulators of ISCs, we carried out a screen in which a collection of transgenic RNAi lines from the Vienna Drosophila RNAi Center and the Bloomington Stock Center ( Dietzl et al., 2007 ; Ni et al., 2009 ) were crossed with act-Gal4, esg-lacZ; Tub-Gal80 ts (referred to as act ts , esg-lacZ ) flies. One-week-old adult flies were shifted to the restrictive temperature (29°C) for 1 week, dissected and stained, and then examined for esg-lacZ -labeled progenitors.

Consumer reviews online have become a major factor in business reputation and brand image due to the popularity of TripAdvisor, Yelp, and online review websites. A negative review can damage the reputation of a business and this has created a new industry of Outlet Pay With Paypal Manchester kriywen 2018 Slippers Glitter Bling Party Woman High Heels Sale High Quality Discount Get Authentic Clearance Classic 3kbnp
where companies attempt to remove or hide bad reviews so that more favourable content is found when potential customers do research.

An expert review usually refers to a review written by someone who has tested several peer products or services to identify which offers the best value for money or the best set of features. An example of this is Amazon Vine. Amazon Vine is a program which was introduced to "help their fellow customers make informed purchase decisions". [3] This program is invite-only and is designed to generate reviews for product vendors that Amazon works with.

One type of user review can be in the physical world, such as a video reviewing a product or software. This is common on platforms such as YouTube.

A bought review is the system where the creator (usually a company) of a new product pays a reviewer to review his new product.

A [book review] (or book report) is a form of criticism in which a book is analyzed based on content, style, and merit. It is often carried out in periodicals, as school work, or online. Its length may vary from a single paragraph to a substantial [essay]. In the case of a work of poetry or fiction, or of nonfiction in which the literary merits of the work are an important element, a review will commonly use the methods of Cheap New Styles Recommend Discount KUIDFAR Wedges Vulcanize Shoes Sneakers Casual Shoes Buy Cheap Release Dates Hmhll
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work. At the other end of the spectrum, some book reviews resemble simple plot summaries. Reviews of non-fiction works intended for instructional or informational purposes may focus more directly on concerns such as practical usefulness and reader-friendliness.

Reviews of live music performances are typically short articles that tell readers about the performers or group(s) that were involved and the pieces or songs that were performed. The comments made by reviewers fall, roughly into two categories: technical comments and subjective/artistic comments. The elements in the "technical" category include rhythmic "togetherness", intonation, errors or slip-ups, and so on. These elements are fairly "black and white"; a pianist playing a concerto either played the right notes on a climactic scale run, or she missed it. The subjective comments refer to elements which are a matter of taste. The balance between the different elements in a review (information about the performer or group; information about the pieces/songs; commentary about the technical and subjective elements of the performance) depends on the audience that a music critic is writing for. Music reviewers writing in local newspapers or general-interest magazines may not be able to assume that the readers will be familiar with music performers and pieces/songs, so they may decide to include a great deal of "background" information.

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"daughter" in English

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"daughter" in American English

daughter noun [ C ]

a female child in relation to her parents :

We have a and a daughter.

Examples for 'daughter'

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Translations of “daughter”

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